Radiography Subtraction Operation
Digital subtraction is a method to subtract and highlight the differences between two source images of the same object. In our case, the two images are two radiographs, e.g. of a tooth taken over a time interval. Radiograph registration, match and overlay is based on the selection of certain landmark (feature) points on both images by the doctor. The doctor is prompted to click on similar landmark (feature) points on the two radiographs. Many digital radiograph images can be opened at the same time, but the doctor must choose the two of them he is going to use. It is recommended to open only two digital radiograph images that will be subtracted subsequently in order to avoid confusion. A magnified view of the area around the current mouse position and the current mouse co-ordinates are shown to make the landmark point selection easier. The software automatically refines landmark point match, corrects geometric distortions between the two radiographs (rotation, translation, scaling), subtracts the two radiographs and presents the result to the doctor for evaluation. The software is user-friendly, runs on any PC and can be operated with minimal computer knowledge.

Pseudocolor Operation
This operation is useful to highlight the interesting areas in the result of the radiograph subtraction. Here the user is prompted to define the grayscale range in which he will apply pseudocoloring, by clicking two pixels in the source image. The pseudocolor operation will be applied in the pixels that have intensity values in the range that was defined by the intensity of the two selected pixels. The user can choose the colour that is going to be used in pseudocoloring. Furthermore, he has the option to apply this operation many times on the same image.

Histogram Equalization Operation
This operation applies a histogram equalization on the result of the radiograph difference. Histogram equalization performs contact enchantment (as well as undesired noise amplification) in an image. This is useful to highlight the interesting areas in the result of the radiography subtraction or in any other image. The only thing that the user has to do is to select the image in which he wants to apply the histogram equalization.

Implementation
EIKONA SUBTRACTION RADIOGRAPHY is a multiple document interface (MDI) Windows (Win32 architecture) application. It is totally coded in C++ programming language by making an extensive use of MFC libraries. All operations are executed through menu commands and dialogs which ensure the user-friendliness of its user interface. Each digital image is displayed as an independent window frame (document) inside the application workspace. A subtraction or pseudocolor operation is executed on the displayed image that is selected by the user.

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Platform Availability
	Microsoft Windows 95/98/Me/NT 4.0/2000/XP

Current Version

4.0

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